Services

Flow Cytometry

Consider RPCI for your Flow Cytometry Needs...

Here is why:

  • STAT results in 4 hours (FOB LFC), non-STAT report in 24 hours.
  • Report includes interpretation and actual pictures of relevant cell populations.
  • Comprehensive service at negotiated price. Special requests at the same price as regular service.
  • All tests use FDA approved Analyte Specific Reagents (ASRs) required for all clinical tests after November 23, 1998.
  • Laboratory certified by DOH and CLIA, approved by JCAHO and CAP.
  • An Internationally recognized laboratory in Clinical Flow Cytometry.

IMAGE CYTOMETRY

  • Full service transmission and scanning electron microscopy
  • Temperature-, CO2- and humidity-controlled multispectral confocal time lapse imaging
  • Full service confocal microcopy
  • Multispectral imaging cytometry for high throughput quantitative imaging 

LUMINEX MULTIPLEX ASSAYS

Soluble Bead Arrays (SBA)

The Luminex 100 platform is used to acquire data from soluble bead arrays (SBA) or multiplexed assays using a capture bead system developed by the Luminex Corporation. An excellent tutorial explaining how the Luminex system works can be found at the corporate website www.luminexcorp.com. Based on a dye color coded scheme up to 100 separate tests can be conducted on a single drop of plasma, serum, lysates or other biological samples. Each bead set will have a separate capture reagent attached to the surface that is directed against a single analyte. Depending upon what analyte is being screened one to several bead sets are incubated with the sample in order capture the analytes. Because of the special dye ratio incorporated each bead, each unique bead population can be analyzed separately after acquisition. Currently, we are screening for cytokines and chemokines from human and mouse samples although the procedure is not limited to these analytes. The bead populations are detected using a 635 nm laser and they are identified based on the ratio of proprietary dyes found in the beads. The captured analytes are detected using a 532 nm laser which is used to excite the fluorochrome phycoerythrin (PE) that is attached to a detection reagent. In the case of cytokines and chemokines a PE labeled antibody directed against the analyte is used as the detection reagent.